X-ray structural analysis of carbonic anhydrase fromChlamydomonas reinhardtii
نویسندگان
چکیده
منابع مشابه
Structural study of X-ray induced activation of carbonic anhydrase.
Carbonic anhydrase, a zinc metalloenzyme, catalyzes the reversible hydration of carbon dioxide to bicarbonate. It is involved in processes connected with acid-base homeostasis, respiration, and photosynthesis. More than 100 distinct human carbonic anhydrase II (HCAII) 3D structures have been generated in last 3 decades [Liljas A, et al. (1972) Nat New Biol 235:131-137], but a structure of an HC...
متن کاملPeriplasmic carbonic anhydrase structural gene (Cah1) mutant in chlamydomonas reinhardtii
To survive in various conditions of CO2 availability, Chlamydomonas reinhardtii shows adaptive changes, such as induction of a CO2-concentrating mechanism, changes in cell organization, and induction of several genes, including a periplasmic carbonic anhydrase (pCA1) encoded by Cah1. Among a collection of insertionally generated mutants, a mutant has been isolated that showed no pCA1 protein an...
متن کاملX-ray crystallography-promoted drug design of carbonic anhydrase inhibitors.
1-N-Alkylated-6-sulfamoyl saccharin derivatives were prepared and assayed as carbonic anhydrase inhibitors (CAIs). During X-ray crystallographic experiments an unexpected hydrolysis of the isothiazole ring was evidenced which allowed us to prepare highly potent enzyme inhibitors with selectivity for some isoforms with medical applications.
متن کاملIdentification of Extracellular Carbonic Anhydrase of Chlamydomonas reinhardtii.
We have examined the induction of carbonic anhydrase activity in Chlamydomonas reinhardtii and have identified the polypeptide responsible for this activity. This polypeptide was not synthesized when the alga was grown photoautotrophically on 5% CO(2), but its synthesis was induced under low concentrations of CO(2) (air levels of CO(2)). In CW-15, a mutant of C. reinhardtii which lacks a cell w...
متن کاملDeciphering proteolytic processing of carbonic anhydrase 1 from Chlamydomonas reinhardtii
iii GENERAL INTRODUCTION 1 PART 1. DECIPHERING PROTEOLYTIC PROCESSING OF PERIPLASMIC CARBONIC ANHYDRASE 1(CAH1) 9
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2005
ISSN: 0108-7673
DOI: 10.1107/s0108767305090355